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Enzyme immunoassays (EIAs) use the catalytic properties of enzymes to detect and quantify immunologic reactions. Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses. In this type of assay, one of the reaction components is nonspecifically adsorbed or covalently bound to the surface of a solid phase, such as a microtiter well, a magnetic particle, or a plastic bead. This attachment facilitates the separation of bound and free-labeled reactants. In
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Future Biotechnology on Instagram: "ELISA, or Enzyme-Linked Immunosorbent Assay, is a widely used laboratory technique to detect and quantify specific proteins, hormones, or antibodies in a sample. The process begins by coating a microplate with an antigen or antibody of interest. A blocking step follows to prevent non-specific binding, which helps improve accuracy. The sample is then added, allowing the target molecule to bind to the coated surface. Next, a primary antibody binds specifically t
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