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9:03
jove.com
HeLa Based Cell Free Expression Systems for Expression of Plasmodium Rhoptry Proteins
11.9K Views. Cleveland State University. Expression of malarial proteins in cell based systems remains challenging. We demonstrate two step and one step IVT (in vitro translation) cell free expression systems for expressing malarial recombinant rhoptry proteins from HeLa cells. We use a Ni-resin affinity based purification system to purify the ...
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