The preparation of standards, also referred to as reference samples, of known concentrations is a frequently used procedure in most analytical laboratories. Internal or external standards with ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; 4,000-cP viscosity; Aldrich); prepare 24 h before use as an aqueous 2% (wt/vol) stock solution by ...

This article presents a simple and systematic approach to HPLC method development, beginning with sample preparation and finishing with practical analytical method validation. step 5 - method ...

Liquid chromatography (LC) is a chromatographic technique used to separate and analyze mixtures of chemical components in solution, to determine if a specific component is present or absent and, if ...

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The majority of biochemical processes are induced and controlled by post-translational modifications on certain proteins. One of the most frequent modifications is phosphorylation of the amino ...