Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...

Reduce amplification bias caused by variable miRNA abundance using automated PCR normalization for NEXTFLEX small RNA sequencing workflows.

PCR has allowed scientists to amplify scarce amounts of DNA and engage in the type of research that will help us understand the genetics of who we are, why people are prone to developing certain ...

Despite its utility, PCR’s main limitation is that it requires precise cycles of heating and cooling to amplify DNA. The thermal cyclers that perform this operation are clunky, relatively expensive, ...